Comparing the cytotoxic potential of Withania somnifera water and methanol extracts

The plant Withania somnifera (Linn.) (Solanacea) is a well-known herbal medicine used in many parts of the world. It has anti-inflammatory, antioxidant, and antitumor as well as neural protective properties. It seems as if the two most active withanolide components, namely withaferin A and withanolide D, found in methanol (MeOH) extracts, are responsible for the anti-inflammatory and antioxidant properties of the plant. The current research evaluated and compared the cytotoxic potential of water and methanol extracts of W. somnifera using a combined crystal violet MTT and Neutral Red assay. MRC-5 cells, a human embryonic lung-derived diploid fibroblast cell line, were the cells of choice. We found that the three lowest concentrations (0.007, 0.042, 0.250 μg/ml) of the plant material extracted in double distilled H2O and MeOH do not differ significantly in any of the assays. We therefore that low concentrations of MeOH extracts (up to 0.250 μg/ml plant material) do not cause cell damage to the MRC-5 cells, however, higher levels should be avoided as cell viability and cell numbers are negatively influenced.Key words: Withania somnifera, anti-inflammatory; water extract; methanol extrac

Comparisons of binary black hole merger waveforms

This a particularly exciting time for gravitational wave physics. Ground-based gravitational wave detectors are now operating at a sensitivity such that gravitational radiation may soon be directly detected, and recently several groups have independently made significant breakthroughs that have finally enabled numerical relativists to solve the Einstein field equations for coalescing black-hole binaries, a key source of gravitational radiation. The numerical relativity community is now in the position to begin providing simulated merger waveforms for use by the data analysis community, and it is therefore very important that we provide ways to validate the results produced by various numerical approaches. Here, we present a simple comparison of the waveforms produced by two very different, but equally successful approaches--the generalized harmonic gauge and the moving puncture methods. We compare waveforms of equal-mass black hole mergers with minimal or vanishing spins. The results show exceptional agreement for the final burst of radiation, with some differences attributable to small spins on the black holes in one case.Comment: Revtex 4, 5 pages. Published versio

The Interpretations For the Low and High Frequency QPO Correlations of X-ray Sources Among White Dwarfs, Neutron Stars and Black Holes

It is found that there exists an empirical linear relation between the high frequency \nhigh and low frequency \nlow of quasi-periodic oscillations (QPOs) for black hole candidate (BHC), neutron star (NS) and white dwarf (WD) in the binary systems, which spans five orders of magnitude in frequency. For the NS Z (Atoll) sources, $\nu_{high}$ and $\nu_{low}$ are identified as the lower kHz QPO frequency and horizontal branch oscillations (HBOs) \nh (broad noise components); for the black hole candidates and low-luminosity neutron stars, they are the QPOs and broad noise components at frequencies between 1 and 10 Hz; for WDs, they are the ``dwarf nova oscillations'' (DNOs) and QPOs of cataclysmic variables (CVs). To interpret this relation, our model ascribes $\nu_{high}$ to the Alfv\'en wave oscillation frequency at a preferred radius and $\nu_{low}$ to the same mechanism at another radius. Then, we can obtain \nlow = 0.08 \nhigh and the relation between the upper kHz QPO frequency \nt and HBO to be \nh \simeq 56 ({\rm Hz}) (\nt/{\rm kHz})^{2}, which are in accordance with the observed empirical relations. Furthermore, some implications of model are discussed, including why QPO frequencies of white dwarfs and neutron stars span five orders of magnitude in frequency. \\Comment: 11 pages, 1 figure, accepted by PAS

Quasi-Spherical Light Cones of the Kerr Geometry

Quasi-spherical light cones are lightlike hypersurfaces of the Kerr geometry that are asymptotic to Minkowski light cones at infinity. We develop the equations of these surfaces and examine their properties. In particular, we show that they are free of caustics for all positive values of the Kerr radial coordinate r. Useful applications include the propagation of high-frequency waves, the definition of Kruskal-like coordinates for a spinning black hole and the characteristic initial-value problem.Comment: LaTeX, 14 pages, 2 figure

Testing outer boundary treatments for the Einstein equations

Various methods of treating outer boundaries in numerical relativity are compared using a simple test problem: a Schwarzschild black hole with an outgoing gravitational wave perturbation. Numerical solutions computed using different boundary treatments are compared to a `reference' numerical solution obtained by placing the outer boundary at a very large radius. For each boundary treatment, the full solutions including constraint violations and extracted gravitational waves are compared to those of the reference solution, thereby assessing the reflections caused by the artificial boundary. These tests use a first-order generalized harmonic formulation of the Einstein equations. Constraint-preserving boundary conditions for this system are reviewed, and an improved boundary condition on the gauge degrees of freedom is presented. Alternate boundary conditions evaluated here include freezing the incoming characteristic fields, Sommerfeld boundary conditions, and the constraint-preserving boundary conditions of Kreiss and Winicour. Rather different approaches to boundary treatments, such as sponge layers and spatial compactification, are also tested. Overall the best treatment found here combines boundary conditions that preserve the constraints, freeze the Newman-Penrose scalar Psi_0, and control gauge reflections.Comment: Modified to agree with version accepted for publication in Class. Quantum Gra

Reducing orbital eccentricity in binary black hole simulations

Binary black hole simulations starting from quasi-circular (i.e., zero radial velocity) initial data have orbits with small but non-zero orbital eccentricities. In this paper the quasi-equilibrium initial-data method is extended to allow non-zero radial velocities to be specified in binary black hole initial data. New low-eccentricity initial data are obtained by adjusting the orbital frequency and radial velocities to minimize the orbital eccentricity, and the resulting ($\sim 5$ orbit) evolutions are compared with those of quasi-circular initial data. Evolutions of the quasi-circular data clearly show eccentric orbits, with eccentricity that decays over time. The precise decay rate depends on the definition of eccentricity; if defined in terms of variations in the orbital frequency, the decay rate agrees well with the prediction of Peters (1964). The gravitational waveforms, which contain $\sim 8$ cycles in the dominant l=m=2 mode, are largely unaffected by the eccentricity of the quasi-circular initial data. The overlap between the dominant mode in the quasi-circular evolution and the same mode in the low-eccentricity evolution is about 0.99.Comment: 27 pages, 9 figures; various minor clarifications; accepted to the "New Frontiers" special issue of CQ

Abacavir: its use and hypersensitivity

Abacavir, a nucleoside reverse transcriptase inhibitor, is useful in first- and second-line HIV therapy and as a substitute for stavudine and zidovudine when toxicity is a problem. Although it is safe and well tolerated, a  lifethreatening hypersensitivity reaction can occur. The risk for developing this reaction relates to the presence of specific genotypes, especially HLA-B*5701

Modeling the Distance-Dependent Blurring in Transmission Imaging in the Ordered-Subset Transmission (OSTR) Algorithm by Using an Unmatched Projector/Backprojector Pair

In SPECT, accurate emission reconstruction requires attenuation compensation with high-quality attenuation maps. Resolution loss in transmission maps could cause blurring and artifacts in emission reconstruction. For a transmission system employing parallel-hole collimators and a sheet source, distance-dependent blurring is caused by the non-ideal source and camera collimations, and the finite intrinsic resolution of the detector. These can be approximately modeled by an incremental-blurring model. To compensate for this blurring in iterative transmission reconstruction, we incorporated the incremental blurring model in the forward projector of the OSTR algorithm but did not include the blur in the backprojector. To evaluate our approach, we simulated transmission projections of the MCAT phantom using a ray-tracing projector, in which the rays coming out from a source point form a narrow cone. The geometric blurring due to the non-ideal source and camera collimations was simulated by multiplying the counts along each cone-beam ray with a weight calculated from the overall geometric response function (assumed a two-dimensional Gaussian function), and then summing the weighted counts into projections. The resulting projections were convolved with the intrinsic response (another two-dimensional Gaussian) to simulate the total system blurring of transmission imaging. Poisson noise was then added to the projection data. We also acquired two sets of transmission measurements of an air-filled Data Spectrum Deluxe SPECT phantom on a Prism 2000 scanning-line-source transmission system. We reconstructed the simulations using the OSTR algorithm, with and without modeling of the incremental blur in the projector. The scaling parameter of the penalty prior was optimized in each case by minimizing the root-mean-square error (RMSE). Reconstructions showed that modeling the incremental blur improved the resolution of the attenuation map and quantitative accuracy.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/85829/1/Fessler211.pd

Estimation of the rigid-body motion from three-dimensional images using a generalized center-of-mass points approach

We present an analytical method for the estimation of rigid-body motion in sets of three-dimensional (3-D) SPECT and PET slices. This method utilizes mathematically defined generalized center-of-mass points in images, requiring no segmentation. It can be applied to compensation of the rigid-body motion in both SPECT and PET, once a series of 3-D tomographic images are available. We generalized the formula for the center-of-mass to obtain a family of points comoving with the object\u27s rigid-body motion. From the family of possible points we chose the best three points which resulted in the minimum root-mean-square difference between images as the generalized center-of-mass points for use in estimating motion. The estimated motion was used to sum the sets of tomographic images, or incorporated in the iterative reconstruction to correct for motion during reconstruction of the combined projection data. For comparison, the principle-axes method was also applied to estimate the rigid-body motion from the same tomographic images. To evaluate our method for different noise levels, we performed simulations with the MCAT phantom. We observed that though noise degraded the motion-detection accuracy, our method helped in reducing the motion artifact both visually and quantitatively. We also acquired four sets of the emission and transmission data of the Data Spectrum Anthropomorphic Phantom positioned at four different locations and/or orientations. From these we generated a composite acquisition simulating periodic phantom movements during acquisition. The simulated motion was calculated from the generalized center-of-mass points calculated from the tomographic images reconstructed from individual acquisitions. We determined that motion-compensation greatly reduced the motion artifact. Finally, in a simulation with the gated MCAT phantom, an exaggerated rigid-body motion was applied to the end-systolic frame. The motion was estimated from the end-diastolic and end-systolic images, and used to sum them into a summed image without obvious artifact. Compared to the principle-axes method, in two of the three comparisons with anthropomorphic phantom data our method estimated the motion in closer agreement to the Polaris system than the principal-axes method, while the principle-axes method gave a more accurate estimation of motion in most cases for the MCAT simulations. As an image-driven approach, our method assumes angularly com plete data sets for each state of motion. We expert this method to be applied in correction of respiratory motion in respiratory gated SPECT, and respiratory or other rigid-body motion in PET. © 2006 IEEE